Twenty-six strains of Candida albicans isolated from various clinical specimens were tested for antifungal susceptibility ot 5 drugs, amphotericin B (AMB), nystatin(NST), clotrimazole(CTZ), ketoconazole(KCZ) and flucytosine(5-FC).
The range of minimum inhibitory concentration (MIC) of the drugs were as follows : AMB, 0.251㎍/ml ; NST, 8-32㎍/ml ; CTZ, <0.125-4㎍/ml ; KCZ, <0.125->128㎍/ml ; 5-FC, 8->1024㎍/ml. 90% MICs of the drugs to Candida albicans were 0.47㎍/ml for
AMB
1.9㎍/ml for CTZ, 1.49㎍/ml for NST, >128㎍/ml for KCZ and >1024㎍/ml for 5-FC.
Restriction enzyme analysis (REA) using restriction endonuclease BamHI performed to char acterized the genomic DNA of C. albicans in molecular aspect.
The resulting gel patterns of REA revealed five intensely stained bands with ethidum bromide (8.7Kb, 7.6Kb, 6.6Kb, 6.0Kb, 3.6Kb). These intense band patterns produced three strain types (group A, B and C). Group A had 8.7Kb, 6.6Kb, 3.6Kb bands.
Group B
had 8.7Kb, 6.0Kb, 3.6Kb bands and group C had 8.7Kb, 3.6Kb bands. Among the total 26 strains, 21 strains belonged to group A. Group B had 3 strains and group C consisted of 2 strains.
In order to elucidate the ras oncogene expression in C. albicans, southern of BamHI digested C. clbicans genomic DNA with α-E32P-labeled v-Ha-ra probe prepared by nick translation was performed. The v-Ha-ras probe was hybridized to C. albicans
genomic
DNA at 4.9kb.
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